Received Apr 26; Accepted Jun 8.
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This article has been cited by other articles in PMC. B Cam zap pussy cells infected with WT or N74D HIV-1 vectors were exposed to the indicated concentrations of digoxin and analysed by flow cytometry 48 hours later.
Lines connect the same donor. Two separate viral stocks were tested. JPG ppat.
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I Cells infected in parallel were analysed by flow cytometry 48h and 10 days post-infection to confirm the effect of raltegravir. Thirty-six hours post-infection, nucleic acids were extracted and used for RNAseq or integration targeting analyses.
Thirty-six hours post-infection, nucleic acids were extracted and used for RNAseq. Thirty-six hours post-infection, DNA was extracted, sheared and integration sites quantified using linker-mediated PCR and deep sequencing. Total clones—the total of unique integration sites.
Shear Sites—the total of proviruses detected across all unique integration sites. Edison local girls duplicates—total of sequencing re detected across all unique integration sites. Each bar in the bar plots describes the of an independent experiment.
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Grey dashed line describes the random expectation using in silico generated integration site Files. B Plots showing integration within genes.
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C Focusing on those integrations within host genes, plots showing proviral orientation relative to the transcriptional start site of cellular genes. Continuous lines indicate direct and experimentally validated interactions between genes; dashed lines indicate experimentally validated, indirect interactions. Cells were labeled with anti-CD38 FITC-conjugated antibodies, stained for cell viability and analyzed by flow cytometry.
Soul mate dating reading genes are susceptible to down regulation by digoxin.
Upon digoxin treatment, the chromatin surrounding these genes becomes repressed, and the provirus is also repressed in tandem.
Digoxin reveals a functional connection between HIV-1 integration preference and T-cell activation
Silencing of the N74D virus is less likely due to its different integration targeting. The values corresponding to each compound are indicated and color-coded. XLSX ppat. Data was de-multiplexed using bcl2fastq v 2. Read QC reports were generated in Illumina Basespace.
Sequencing re were aligned to an appropriate reference genome using TopHat v2. XLS ppat.
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Prepared sequencing libraries were sequenced on the Illumina HiSeq platform. These compounds were not validated.
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